ShineGAL4 drivers for tissue and cell-type specific optogenetics in Drosophila

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Victor Girard Sebastian Sorge Joachim Kurth Cyrille Alexandre Alex Gould
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Abstract

An optogenetic split-GAL4 system, ShineGAL4, allows genes to be manipulated with unprecedented spatiotemporal precision. Here, we convert a panel of 14 GAL4 drivers widely used in Drosophila research into their ShineGAL4 counterparts. Homology assisted CRISPR knock-in (HACK) is used to replace GAL4 with the GAL4 DNA binding domain fused to a Magnet photoswitch. We show that the resulting ShineGAL4 drivers enable gene expression to be rapidly induced by light specifically in fat body, muscles, enterocytes, oenocytes, Malpighian tubules, neurons, neuroblast lineages, glial subtypes or in all glia. We also develop an optogenetic cassette for photoactivation of GAL4 in 'silent' FLP-out clones. This panel of optogenetic tools will enable precise spatiotemporal control of gene expression in a wide range of different Drosophila tissues and cell-types.

Journal details

Journal Development
Volume 153
Issue number 4
Pages dev204981
Available online
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Publisher website (DOI) 10.1242/dev.204981
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Europe PubMed Central 41738557
Pubmed 41738557

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