Multi-contrast X-ray microtomography of human lung specimens with an extended field-of-view
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Harry Allan Adam Doherty Carlos Navarrete-León Oriol Roche I Morgó Yunpeng Jia Charlotte Percival Zoe Hagel Kate EJ Otter Chuen Ryan Khaw Kate HC Gowers Helen Hall Sam M Janes Fleur Monk David Moore Ryoko Egashira Joseph Jacob Marco EndrizziAbstract
BACKGROUND: Phase-based X-ray microtomography is a powerful technique capable of quantitative volumetric imaging of lung tissue in health and disease. The maximum sample size is however limited by the fixed sizes of detectors and optical elements. Thus while high-resolution imaging can offer valuable microscale insights, it can be difficult to interpret without the context of the surrounding tissue. We propose a multi-contrast and multi-scale approach, combined with an offset geometry to extend the field-of-view (FOV). PURPOSE: FOV limitations make it a challenge to simultaneously achieve high spatial-resolution and image large samples. Our method doubles the possible FOV achievable for a given spatial-resolution, in a way compatible with multiple scales and imaging systems. METHODS: Multi-contrast whole sample volumetric images are acquired using a beam-tracking X-ray phase-contrast imaging(XPCI) system. Following this, a section of the same sample is imaged at higher resolution using an X-ray microscope with propagation-based imaging. The FOV of both methods is doubled using an offset center-of-rotation geometry, followed by weighted analytical reconstruction. RESULTS: We present exemplary multi-contrast reconstructions of resected human lung tissue at 10.5 μ m $\umu{\rm m}$ voxel size across a 4.3 cm horizontal FOV, and at 450 nm voxel size for a 2.7 mm section of the same sample. This enables the visualization of a range of features, from the macro to the cellular scale. CONCLUSIONS: We demonstrate a versatile method to image large samples without sacrificing spatial-resolution. This method is directly compatible with complementary implementations of XPCI, and is easily adapted to a range of other systems.
Journal details
Journal
Medical Physics
Volume
53
Issue number
2
Pages
e70335
Available online
Publication date
Full text links
Publisher website (DOI)
10.1002/mp.70335
Figshare
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Europe PubMed Central
41712206
Pubmed
41712206
