Interphase cell morphology defines the mode, symmetry, and outcome of mitosis

Authors list

Holly E Lovegrove Georgia E Hulmes Sabrina Ghadaouia Christopher Revell Marta Giralt-Pujol Zain Alhashem Andreia Pena Damian D Nogare Ellen Appleton Guilherme Costa Richard L Mort Christoph Ballestrem Gareth W Jones Cerys S Manning Ajay B Chitnis Claudio A Franco Claudia Linker Katie Bentley Shane P Herbert
Scroll for detail on labs, facilities & authors

Abstract

During tissue formation, dynamic cell shape changes drive morphogenesis while asymmetric divisions create cellular diversity. We found that the shifts in cell morphology that shape tissues could concomitantly act as conserved instructive cues that trigger asymmetric division and direct core identity decisions underpinning tissue building. We performed single-cell morphometric analyses of endothelial and other mesenchymal-like cells. Distinct morphological changes switched cells to an "isomorphic" mode of division, which preserved pre-mitotic morphology throughout mitosis. In isomorphic divisions, interphase morphology appeared to provide a geometric code defining mitotic symmetry, fate determinant partitioning, and daughter state. Rab4-positive endosomes recognized this code, allowing them to respond to pre-mitotic morphology and segregate determinants accordingly. Thus, morphogenetic shape change sculpts tissue form while also generating cellular heterogeneity, thereby driving tissue assembly.

Journal details

Journal Science
Volume 388
Issue number 6746
Pages eadu9628
Available online
Publication date

Full text links

Publisher website (DOI) 10.1126/science.adu9628
Europe PubMed Central 40310923
Pubmed 40310923

Keywords

Type of publication

Crick labs/facilities

Crick authors

Crick First author
Crick Corresponding author